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basic fibroblast growth factor  (R&D Systems)


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    R&D Systems basic fibroblast growth factor
    Basic Fibroblast Growth Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 905 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+fgf+basic+protein/Recombinant+Human+FGF+basic%2FFGF2%2FbFGF+(146+aa)+Protein/pmc13132546-182-33-37
    Average 96 stars, based on 905 article reviews
    basic fibroblast growth factor - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: Fibrinogen in the glioblastoma microenvironment contributes to the invasiveness of brain tumor-initiating cells.
    Article Snippet: .. This medium was supplemented with NeuroCult Proliferation Supplement (Stemcell Technologies), 20 ng/ml recombinant human FGF basic protein (R&D Systems), 20 ng/ ml recombinant human EGF (Peprotech), and 0.2% Heparin Solution (Stemcell Technologies). ..

    Article Title: Development of Human-Derived Cell Culture Lines for Recurrent Respiratory Papillomatosis
    Article Snippet: Fresh papilloma specimens were collected from patients with active papilloma ( ) undergoing surgical debridement and control specimens were obtained from children undergoing routine tonsillectomy. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Case Number HPV Type In vitro Cell growth Number of surgeries Sex Ages Diagnosis 1 6 Y 70 F 15.3 Papillomatosis of larynx 2 6 Y 50 M 16.5 Papillomatosis of larynx 3 11 N 68 M 12.5 Papillomatosis of trachea 4 6 N 29 F 25.5 Papillomatosis of larynx 5 11 Y 92 F 23.5 Papilloma of larynx 6 6 Y 21 F 4.7 Recurrent glottic respiratory papillomatosis 7 6 Y 7 F 18.6 Laryngeal papillomatosis 8 11 Y 6 M 2.3 Recurrent respiratory papillomatosis 9 11 Y 3 M 16.4 Papilloma of nasopharynx 10 6 N 21 F 3.5 Recurrent respiratory papillomatosis 11 11 N 21 F 11.0 Recurrent glottic respiratory papillomatosis 12 11 Y 29 F 18.8 Papilloma of nose 13 6 Y 47 M 22.2 Papillomatosis of larynx Open in a separate window Specimen details Tissue samples were minced and digested with Dispase II (5mg/ml; Roche Life Science) and then Trypsin. .. Cells were cultured in EpiLife® Medium (Gibco, ThermoFisher, MA, USA) supplemented with Human Keratinocyte Growth Supplement (Gibco); Bovine Pituitary Extract (Gibco); 10–40 ng/ml Recombinant Human EGF Protein (R&D systems, Minneapolis, MN); 10–30 ng/ml Recombinant Human FGF Basic Protein (R&D systems) and addition of 5–10 umol/L Y-27632 (Enzo Life Sciences, Farmingdale, NY, USA) without feeder cells to facilitate downstream analyses. .. Immunofluorescence Cells grown on coverslips were fixed with 4% formaldehyde, blocked with 3% BSA, incubated in mouse anti-cytokeratin 5 (ab17130; Abcam, Cambridge, MA, USA), rabbit anti-cytokeratin 8+18 (ab53280; Abcam), mouse immunoglobulin (SC-2025; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or rabbit immunoglobulin (#2729; Cell signaling Technology, Beverly, MA, USA), detected with Alexa Fluor 488 anti-mouse IgG ( Life Technologies, Carlsbad, CA, USA) or Alexa Fluor 568 anti-rabbit antibody ( Life Technologies) and mounted with ProLong Gold Antifade Mountant with DAPI (Life Technologies).

    Article Title: Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4
    Article Snippet: Strain, strain background ( Escherichia coli ) , TransStbl3 Chemically Competent Cell , TransGen Biotech , Cat#: CD521 , . .. Peptide, recombinant protein , bFGF, Recombinant Human FGF basic Protein , R and D Systems , Cat#: 233-FB-CF , . .. Chemical compound, drug , G418, Geneticin , Thermo Fisher Scientific , Cat#: 10131035 , .

    Article Title: Fibrinogen in the glioblastoma microenvironment contributes to the invasiveness of brain tumor‐initiating cells
    Article Snippet: .. This medium was supplemented with NeuroCult Proliferation Supplement (Stemcell Technologies), 20 ng/ml recombinant human FGF basic protein (R&D Systems), 20 ng/ml recombinant human EGF (Peprotech), and 0.2% Heparin Solution (Stemcell Technologies). ..

    Article Title: Ammonia Induces a Myostatin-Mediated Atrophy in Mammalian Myotubes, but Induces Hypertrophy in Avian Myotubes
    Article Snippet: Chicken and C2C12 myoblasts were cultured in proliferation media consisting of Dulbecco’s Modified Eagle’s Medium without L-glutamine (DMEM, Sigma Aldrich), 15% fetal bovine serum (FBS, Genclone, San Diego, CA), 1% Antibiotic Antimycotic Solution (Sigma Aldrich), 1mM Lglutamine (L-Glutamine, Gibco, Burlington, Ontario, Canada). .. Proliferation media for rat myoblasts consisted of HamsF10 media (F10, Sigma Aldrich), 20% FBS (Genclone), 1% Antibiotic Antimycotic solution (Sigma Aldrich), and 2.5 ng/mL Recombinant Human FGF basic protein (R&D Systems, Minneapolis, MN). .. Myoblast differentiation media for all cell types contained DMEM without L-glutamine, 10% horse serum Frontiers in Sustainable Food Systems | www.frontiersin.org 2 December 2019 | Volume 3 | Article 115 (Hyclone, Logan, UT), 1% Antibiotic Antimycotic Solution (Sigma Aldrich), 1mM L-glutamine (Gibco).

    Article Title: Epitranscriptomic editing of the RNA N6-methyladenosine modification by dCasRx conjugated methyltransferase and demethylase
    Article Snippet: HEK293T cells were cultured in Dulbecco's Modified Eagle Medium (Gibco, #C11995500CP) supplemented with 10% fetal bovine serum (Gibco, #10099-141C), 1% penicillin/streptomycin (Hyclone, #SV30010) and 1% GlutaMaxTM Supplement (Gibco, #35050-061) at 37°C, 5% CO 2 . .. GSC 3565 and GSC 468 cells were cultured in NeurobasalTM-A Medium (Gibco, #12349-015) supplemented with 2% B-27TM supplement (Gibco, #12587-010), 20 ng/ml recombinant human EGF protein (R&D, #236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, #4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco, #11360-070) and 1% GlutaMaxTM supplement (Gibco, #35050-061) at 37°C, 5% CO 2 . .. Plasmid transfection was carried out using LipoD293TM In Vitro DNA Transfection Reagent (SignaGen Laboratories, SL100668) following manufacture's protocol.

    Article Title: Epitranscriptomic editing of the RNA N6-methyladenosine modification by dCasRx conjugated methyltransferase and demethylase
    Article Snippet: HEK293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, # C11995500CP) supplemented with 10% fetal bovine serum (FBS, Gibco, # 10099-141C), 1% penicillin/streptomycin (Hyclone # SV30010), and 1% GlutaMaxTM Supplement (Gibco, # 35050-061) at 37°C, 5% CO2. .. GSC 3565 and GSC 468 cells were cultured in NeurobasalTM-A Medium (Gibco, # 12349-015) supplemented with 1% B-27TM supplement (Gibco, # 12587-010), 20 ng/ml recombinant human EGF protein (R&D, # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco, # 11360-070), and 1% GlutaMaxTM supplement (Gibco, # 35050-061) at 37°C, 5% CO2. .. Plasmid transfection was carried out using LipoD293TM In Vitro DNA Transfection Reagent (SignaGen Laboratories, SL100668) following manufacture's protocol.

    Cell Culture:

    Article Title: Development of Human-Derived Cell Culture Lines for Recurrent Respiratory Papillomatosis
    Article Snippet: Fresh papilloma specimens were collected from patients with active papilloma ( ) undergoing surgical debridement and control specimens were obtained from children undergoing routine tonsillectomy. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Case Number HPV Type In vitro Cell growth Number of surgeries Sex Ages Diagnosis 1 6 Y 70 F 15.3 Papillomatosis of larynx 2 6 Y 50 M 16.5 Papillomatosis of larynx 3 11 N 68 M 12.5 Papillomatosis of trachea 4 6 N 29 F 25.5 Papillomatosis of larynx 5 11 Y 92 F 23.5 Papilloma of larynx 6 6 Y 21 F 4.7 Recurrent glottic respiratory papillomatosis 7 6 Y 7 F 18.6 Laryngeal papillomatosis 8 11 Y 6 M 2.3 Recurrent respiratory papillomatosis 9 11 Y 3 M 16.4 Papilloma of nasopharynx 10 6 N 21 F 3.5 Recurrent respiratory papillomatosis 11 11 N 21 F 11.0 Recurrent glottic respiratory papillomatosis 12 11 Y 29 F 18.8 Papilloma of nose 13 6 Y 47 M 22.2 Papillomatosis of larynx Open in a separate window Specimen details Tissue samples were minced and digested with Dispase II (5mg/ml; Roche Life Science) and then Trypsin. .. Cells were cultured in EpiLife® Medium (Gibco, ThermoFisher, MA, USA) supplemented with Human Keratinocyte Growth Supplement (Gibco); Bovine Pituitary Extract (Gibco); 10–40 ng/ml Recombinant Human EGF Protein (R&D systems, Minneapolis, MN); 10–30 ng/ml Recombinant Human FGF Basic Protein (R&D systems) and addition of 5–10 umol/L Y-27632 (Enzo Life Sciences, Farmingdale, NY, USA) without feeder cells to facilitate downstream analyses. .. Immunofluorescence Cells grown on coverslips were fixed with 4% formaldehyde, blocked with 3% BSA, incubated in mouse anti-cytokeratin 5 (ab17130; Abcam, Cambridge, MA, USA), rabbit anti-cytokeratin 8+18 (ab53280; Abcam), mouse immunoglobulin (SC-2025; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or rabbit immunoglobulin (#2729; Cell signaling Technology, Beverly, MA, USA), detected with Alexa Fluor 488 anti-mouse IgG ( Life Technologies, Carlsbad, CA, USA) or Alexa Fluor 568 anti-rabbit antibody ( Life Technologies) and mounted with ProLong Gold Antifade Mountant with DAPI (Life Technologies).

    Article Title: Epitranscriptomic editing of the RNA N6-methyladenosine modification by dCasRx conjugated methyltransferase and demethylase
    Article Snippet: HEK293T cells were cultured in Dulbecco's Modified Eagle Medium (Gibco, #C11995500CP) supplemented with 10% fetal bovine serum (Gibco, #10099-141C), 1% penicillin/streptomycin (Hyclone, #SV30010) and 1% GlutaMaxTM Supplement (Gibco, #35050-061) at 37°C, 5% CO 2 . .. GSC 3565 and GSC 468 cells were cultured in NeurobasalTM-A Medium (Gibco, #12349-015) supplemented with 2% B-27TM supplement (Gibco, #12587-010), 20 ng/ml recombinant human EGF protein (R&D, #236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, #4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco, #11360-070) and 1% GlutaMaxTM supplement (Gibco, #35050-061) at 37°C, 5% CO 2 . .. Plasmid transfection was carried out using LipoD293TM In Vitro DNA Transfection Reagent (SignaGen Laboratories, SL100668) following manufacture's protocol.

    Article Title: Epitranscriptomic editing of the RNA N6-methyladenosine modification by dCasRx conjugated methyltransferase and demethylase
    Article Snippet: HEK293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, # C11995500CP) supplemented with 10% fetal bovine serum (FBS, Gibco, # 10099-141C), 1% penicillin/streptomycin (Hyclone # SV30010), and 1% GlutaMaxTM Supplement (Gibco, # 35050-061) at 37°C, 5% CO2. .. GSC 3565 and GSC 468 cells were cultured in NeurobasalTM-A Medium (Gibco, # 12349-015) supplemented with 1% B-27TM supplement (Gibco, # 12587-010), 20 ng/ml recombinant human EGF protein (R&D, # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco, # 11360-070), and 1% GlutaMaxTM supplement (Gibco, # 35050-061) at 37°C, 5% CO2. .. Plasmid transfection was carried out using LipoD293TM In Vitro DNA Transfection Reagent (SignaGen Laboratories, SL100668) following manufacture's protocol.



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